Abstract
Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cattle
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Cyclic AMP / analogs & derivatives
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Cyclic AMP / metabolism
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Glutathione Transferase / metabolism*
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Glycomics / methods*
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Lectins / metabolism*
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Protein Array Analysis / methods*
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Recombinant Fusion Proteins / metabolism*
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Serum Albumin, Bovine / metabolism
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Surface Properties
Substances
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Lectins
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Recombinant Fusion Proteins
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Serum Albumin, Bovine
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8-aminohexylamino cAMP
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Cyclic AMP
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Glutathione Transferase