Intracellular delivery of RNA-based therapeutics using aptamers

Ther Deliv. 2010 Dec;1(6):849-61. doi: 10.4155/tde.10.61.

Abstract

The clinical potential of siRNAs for silencing genes critical to disease progression is clear, but a fail-proof method for delivering siRNAs to the cytoplasm of diseased tissues or cells has yet to be identified. A variety of delivery approaches have been explored to directly or indirectly couple siRNAs to delivery vehicles. This review explores the use of synthetic single-stranded DNA and RNA aptamers as a means to deliver siRNAs, shRNAs and antisense oligonucleotides for therapeutic intervention. Topics covered include: the advantages and challenges of using aptamers as delivery tools; current aptamer-mediated siRNA delivery platforms for the treatment of cancer and HIV; and emerging methodologies for the identification of aptamers capable of internalizing into target cell types.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Aptamers, Nucleotide / administration & dosage*
  • CD4 Antigens / genetics
  • HIV Envelope Protein gp120 / genetics
  • HIV Infections / drug therapy*
  • Humans
  • Neoplasms / drug therapy*
  • RNA, Small Interfering / administration & dosage*

Substances

  • Aptamers, Nucleotide
  • CD4 Antigens
  • HIV Envelope Protein gp120
  • RNA, Small Interfering
  • gp120 protein, Human immunodeficiency virus 1