Methicillin-resistant Staphylococcus aureus (MRSA) ST398 in a farmer with skin lesions and in pigs of his farm: clonal relationship and detection of lnu(A) gene

Clin Microbiol Infect. 2011 Jun;17(6):923-7. doi: 10.1111/j.1469-0691.2010.03437.x. Epub 2011 Jan 6.

Abstract

Skin infection associated with methicillin-resistant Staphylococcus aureus (MRSA)-ST398 was detected in a pig-farmer, and MRSA-ST398 isolates were also detected in nasal samples of the patient and of 11/12 pigs on his farm. Twelve MRSA isolates were obtained from skin lesions (n = 6) and nasal samples (n = 6) of the patient in two sampling moments and 11 MRSA isolates from nasal samples of pigs. They were typed as t011-SCCmecIVa-agrI and t108-SCCmecV-agrI (patient and pigs) and t588-SCCmecV-agrI (patient). The following resistance genes were detected (number isolates): tet(K) (1), tet(L) (23), tet(M) (13), erm(A) (13), erm(C) (13), msr(A) (11), lnu(A) (21), aph(2'')-acc(6') (3), ant(4') (13), aph(3') (12), dfrS1 (15) and dfrK (22). Seventeen human and animal MRSA-ST398 isolates showed indistinguishable PFGE patterns (A1-spa-t011 or B2-spa-t108) and similar phenotypic-genotypic characteristics, including the presence of the lnu(A) gene, associated with lincomycin resistance. Potential pig-to-human transference of ST398 is suggested in this study. The first detection of the lnu(A) gene in MRSA-ST398 is reported.

Publication types

  • Case Reports

MeSH terms

  • Agriculture
  • Animals
  • Bacterial Typing Techniques
  • Carrier State / microbiology
  • Carrier State / veterinary
  • Cluster Analysis
  • Electrophoresis, Gel, Pulsed-Field
  • Genes, Bacterial
  • Genotype
  • Humans
  • Male
  • Methicillin-Resistant Staphylococcus aureus / classification*
  • Methicillin-Resistant Staphylococcus aureus / genetics
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification*
  • Middle Aged
  • Molecular Epidemiology
  • Molecular Typing
  • Nasal Mucosa / microbiology
  • Staphylococcal Skin Infections / diagnosis*
  • Staphylococcal Skin Infections / microbiology*
  • Swine / microbiology*
  • Zoonoses / microbiology*
  • Zoonoses / transmission*