Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine by a chemically designed peptide

Chem Commun (Camb). 2011 Aug 7;47(29):8277-9. doi: 10.1039/c1cc12131f. Epub 2011 Jun 21.

Abstract

An artificial phosphopeptide recognized the difference between methylated and hydroxymethylated cytosines in DNA. The Sp1 zinc finger peptide substituted by phosphotyrosine effectively discriminated between 5-methylcytosine, 5-hydroxymethylcytosine ((hm)C) and unmethylated cytosine. The DNA recognition properties of the peptide differ from those of other chemicals that detect (hm)C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Methylcytosine / analysis*
  • Cytosine / analogs & derivatives*
  • Cytosine / analysis
  • DNA / chemistry
  • Electrophoretic Mobility Shift Assay / methods*
  • Oxidation-Reduction
  • Phosphopeptides / chemistry*
  • Phosphotyrosine / chemistry
  • Protein Kinases / chemistry
  • Zinc Fingers

Substances

  • Phosphopeptides
  • 5-hydroxymethylcytosine
  • Phosphotyrosine
  • 5-Methylcytosine
  • Cytosine
  • DNA
  • Protein Kinases
  • Sp1 kinase