Inflammatory response in rat lungs with recurrent exposure to welding fumes: a transcriptomic approach

Toxicol Ind Health. 2012 Apr;28(3):203-15. doi: 10.1177/0748233711410906. Epub 2011 Jul 5.

Abstract

As chronic exposure to welding fumes causes pulmonary diseases, such as pneumoconiosis, public concern has increased regarding continued exposure to these hazardous gases in the workplace. In a previous study, the inflammatory response to welding fume exposure was analysed in rat lungs in the case of recurrent exposure and recovery periods. Thus using lung samples, well-annotated by histological observation and biochemical analysis, this study examines the gene expression profiles to identify phenotype-anchored genes corresponding to lung inflammation and the repair phenomenon after recurrent welding fume exposure. Seven genes (Mmp12, Cd5l, LOC50101, LOC69183, Spp1, and Slc26a4) were found to be significantly up-regulated according to the severity of the lung injury. In addition, the transcription and translation of Trem2, which was up-regulated in response to the repair process, were validated using a real-time polymerase chain reaction, Western blotting, and immunohistochemistry. The differentially expressed genes in the exposure and recovery groups were also classified using k-means and hierarchical clustering, plus their toxicological function and canonical pathways were further analysed using Ingenuity Pathways Analysis Software. As a result, this comprehensive and integrative analysis of the transcriptional changes that occur during repeated exposure provides important information on the inflammation and repair processes after welding-fume-induced lung injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Air Pollutants, Occupational / toxicity*
  • Analysis of Variance
  • Animals
  • Blotting, Western
  • Bronchoalveolar Lavage Fluid / chemistry
  • Cluster Analysis
  • Gene Expression Profiling
  • Immunohistochemistry
  • Inhalation Exposure / analysis*
  • Lung / chemistry
  • Lung / drug effects
  • Lung Injury / chemically induced*
  • Lung Injury / immunology
  • Lung Injury / metabolism
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Signal Transduction
  • Transcriptome*
  • Up-Regulation
  • Welding*

Substances

  • Air Pollutants, Occupational