Abstract
Novel circular ssDNA genomes have recently been detected in animals and in the environment using metagenomic and high-throughput sequencing approaches. In this study, five full-length circular ssDNA genomes were recovered from bat faecal samples using inverse PCR with sequences designed based on circovirus-related sequences obtained from Solexa sequencing data derived from a random amplification method. These five sequences shared a similar genomic organization to circovirus or the recently proposed cyclovirus of the family Circoviridae. The newly obtained circovirus/cyclovirus-like genomes ranged from 1741 to 2177 bp, and each consisted of two major ORFs, ORF1 and ORF2, encoding putative replicase (Rep) and capsid (Cap) proteins, respectively. The potential stem-loop region was predicted in all five genomes, and three of them had the typical conserved nonanucleotide motif of cycloviruses. A set of primers targeting the conserved Rep region was designed and used to detect the prevalence of circovirus/cyclovirus sequences in individual bats. Among 199 samples tested, 47 were positive (23.6%) for the circovirus genome and two (1.0%) were positive for the cyclovirus genome. In total, 48 partial Rep sequences plus the five full-length genomes were obtained in this study. Detailed analysis indicated that these sequences are distantly related to known circovirus/cyclovirus genomes and may represent 22 novel species that belong to the family Circoviridae.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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China
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Chiroptera
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Circoviridae / classification*
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Circoviridae / genetics*
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Circoviridae / isolation & purification
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Circoviridae Infections / veterinary*
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Circoviridae Infections / virology
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DNA, Viral / chemistry
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DNA, Viral / genetics*
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Feces / virology
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Genetic Variation*
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Molecular Sequence Data
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Open Reading Frames
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Sequence Analysis, DNA
Associated data
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GENBANK/JF938078
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GENBANK/JF938079
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GENBANK/JF938080
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GENBANK/JF938081
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