Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation

J Clin Microbiol. 2011 Oct;49(10):3691-3. doi: 10.1128/JCM.01018-11. Epub 2011 Aug 3.

Abstract

We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteremia / diagnosis*
  • Bacterial Translocation*
  • Bacteriological Techniques / methods*
  • DNA, Bacterial / blood
  • Genes, rRNA*
  • HIV Infections / complications*
  • Humans
  • Lipopolysaccharides / blood
  • Plasma / chemistry
  • Plasma / microbiology*
  • RNA, Ribosomal, 16S / genetics
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Statistics as Topic

Substances

  • DNA, Bacterial
  • Lipopolysaccharides
  • RNA, Ribosomal, 16S