Harmonin inhibits presynaptic Cav1.3 Ca²⁺ channels in mouse inner hair cells

Nat Neurosci. 2011 Aug 7;14(9):1109-11. doi: 10.1038/nn.2895.

Abstract

Harmonin is a scaffolding protein that is required for normal mechanosensory function in hair cells. We found a presynaptic association of harmonin and Ca(v)1.3 Ca(2+) channels at the mouse inner hair cell synapse, which limits channel availability through a ubiquitin-dependent pathway.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Alcohol Oxidoreductases / metabolism
  • Animals
  • Animals, Newborn
  • Calcium / metabolism
  • Calcium Channels, L-Type / deficiency
  • Calcium Channels, L-Type / metabolism*
  • Carrier Proteins / metabolism*
  • Cell Cycle Proteins
  • Cell Line, Transformed
  • Cytoskeletal Proteins
  • DNA-Binding Proteins / metabolism
  • Ear, Inner / cytology
  • Electric Stimulation
  • Gene Expression Regulation, Developmental / physiology
  • Hair Cells, Auditory, Inner / drug effects
  • Hair Cells, Auditory, Inner / physiology*
  • Humans
  • Immunoprecipitation / methods
  • In Vitro Techniques
  • Ion Channel Gating / genetics
  • Membrane Potentials / drug effects*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • PDZ Domains / drug effects
  • PDZ Domains / physiology
  • Patch-Clamp Techniques
  • Presynaptic Terminals / drug effects*
  • Presynaptic Terminals / physiology*
  • Protein Binding / drug effects
  • Synapses / genetics
  • Synapses / physiology*
  • Transfection
  • Ubiquitin / metabolism

Substances

  • Cacna1d protein, mouse
  • Calcium Channels, L-Type
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cytoskeletal Proteins
  • DNA-Binding Proteins
  • Ubiquitin
  • Ush1c protein, mouse
  • Alcohol Oxidoreductases
  • C-terminal binding protein
  • Calcium