Antiviral activities of sulfated polysaccharides isolated from Sphaerococcus coronopifolius (Rhodophytha, Gigartinales) and Boergeseniella thuyoides (Rhodophyta, Ceramiales)

Mar Drugs. 2011;9(7):1187-1209. doi: 10.3390/md9071187. Epub 2011 Jul 6.

Abstract

Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 μg/mL. In addition, polysaccharides were capable of inhibiting the in vitro replication of Herpes simplex virus type 1 (HSV-1) on Vero cells values of EC₅₀ of 4.1 and 17.2 μg/mL, respectively. The adsorption step of HSV-1 to the host cell seems to be the specific target for polysaccharide action. While for HIV-1, these results suggest a direct inhibitory effect on HIV-1 replication by controlling the appearance of the new generations of virus and potential virucidal effect. The polysaccharides from S. coronopifolius (PSC) and B. thuyoides (PBT) were composed of galactose, 3,6-anhydrogalactose, uronics acids, sulfate in ratios of 33.1, 11.0, 7.7 and 24.0% (w/w) and 25.4, 16.0, 3.2, 7.6% (w/w), respectively.

Keywords: B. thuyoides; Herpes simplex virus; S. coronopifolius; antiviral activity; chemical composition; human immunodeficiency virus; sulfated polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption / drug effects
  • Animals
  • Antiviral Agents / analysis
  • Antiviral Agents / chemistry
  • Antiviral Agents / isolation & purification
  • Antiviral Agents / pharmacology*
  • Cell Survival
  • Chlorocebus aethiops
  • Galactose / analogs & derivatives
  • Galactose / chemistry
  • Galactose / pharmacology
  • HIV-1 / drug effects
  • Herpesvirus 1, Human / drug effects
  • Humans
  • Matrix Metalloproteinase 17 / drug effects
  • Molecular Weight
  • Morocco
  • Oceans and Seas
  • Phytotherapy
  • Plant Preparations / pharmacology
  • Polysaccharides / analysis
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Rhodophyta / chemistry*
  • Sulfates / chemistry
  • Sulfates / pharmacology
  • Time Factors
  • Uronic Acids / chemistry
  • Uronic Acids / pharmacology
  • Vero Cells
  • Virus Replication / drug effects

Substances

  • Antiviral Agents
  • Plant Preparations
  • Polysaccharides
  • Sulfates
  • Uronic Acids
  • 3,6-anhydrogalactose
  • Matrix Metalloproteinase 17
  • Galactose