Effect of implantation site and injury condition on host response to human-derived fascia lata ECM in a rat model

J Orthop Res. 2012 Mar;30(3):461-7. doi: 10.1002/jor.21529. Epub 2011 Aug 19.

Abstract

The host response and remodeling of ECM scaffolds are believed to be critical determinants of success or failure in repair or reconstructive procedures. Host response has been investigated in subcutaneous or abdominal wall implantation models. The extent to which evaluation of the host response to ECM intended for tendon or ligament repair should be performed in an orthotopic site is not known. This study compared the host response to human-derived fascia lata ECM among various implantation sites in the rat model. Results showed that a xenograft in the rat shoulder does not exhibit a different host response at 7 days from xenograft in the body wall, suggesting that either site may be appropriate to study the early host response to biologic grafts as well as the effect of various treatments aimed to modify the early host response. By 28 days, a xenograft in the rat shoulder does elicit a unique host response from that seen in the body wall. Therefore, it may be more appropriate to use an orthotopic shoulder model for investigating the long-term host response and remodeling of biologic grafts to be used for rotator cuff repair.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abdominal Wall / surgery
  • Animals
  • Cytokines / metabolism
  • Extracellular Matrix / metabolism
  • Extracellular Matrix / pathology
  • Extracellular Matrix / transplantation*
  • Fascia Lata / metabolism
  • Fascia Lata / pathology
  • Fascia Lata / transplantation*
  • Gene Expression
  • Humans
  • Inflammation / etiology*
  • Inflammation / pathology
  • Lumbosacral Region / surgery
  • Male
  • Rats
  • Rats, Inbred Lew
  • Shoulder / surgery
  • Tissue Scaffolds*
  • Transplantation, Heterologous / adverse effects*

Substances

  • Cytokines