Abstract
This paper describes a simple and sensitive aptamer/graphene oxide (GO) based assay for insulin detection. GO can protect DNA from nuclease cleavage, but aptamers can be detached from the GO surface by specific target binding. This exposes the aptamers to enzymatic cleavage and releases the target for a new cycle. Cycling of targets leads to significant signal amplification and low LOD.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Aptamers, Nucleotide / chemistry*
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Deoxyribonuclease I / metabolism
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Fluorescent Dyes / chemistry
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Graphite / chemistry*
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Insulin / analysis*
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Oxides / chemistry*
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SELEX Aptamer Technique
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Spectrometry, Fluorescence
Substances
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Aptamers, Nucleotide
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Fluorescent Dyes
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Insulin
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Oxides
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Graphite
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Deoxyribonuclease I