Patterning small-molecule biocapture surfaces: microcontact insertion printing vs. photolithography

Chem Commun (Camb). 2011 Oct 14;47(38):10641-3. doi: 10.1039/c1cc13002a. Epub 2011 Aug 26.

Abstract

Chemical patterns prepared by self-assembly, combined with soft lithography or photolithography, are directly compared. Pattern fidelity can be controlled in both cases but patterning at the low densities necessary for small-molecule probe capture of large biomolecule targets is better accomplished using microcontact insertion printing (μCIP). Surfaces patterned by μCIP are used to capture biomolecule binding partners for the small molecules dopamine and biotin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies / immunology
  • Biotin / chemistry*
  • Dopamine / chemistry*
  • Gold / chemistry
  • Hydrazines / chemistry
  • Microscopy, Fluorescence
  • Rabbits
  • Streptavidin / immunology
  • Surface Properties

Substances

  • Alexa 488 hydrazide
  • Antibodies
  • Hydrazines
  • Biotin
  • Gold
  • Streptavidin
  • Dopamine