Stable precursor fragments of vasoactive peptides in umbilical cord blood of term and preterm infants

Horm Res Paediatr. 2011;76(4):234-9. doi: 10.1159/000329285. Epub 2011 Sep 2.

Abstract

Background: Though various neurohormonal systems are concurrently activated during birth, their biological effectors are not always easy to measure due to their short half-life in vivo, instability in biological samples, or very low concentrations.

Methods: Using a recently discovered chemiluminescence assay, we measured the stable precursor fragments mid-regional pro-atrial natriuretic peptide (MR-proANP), mid-regional pro-adrenomedullin (MR-proADM), C-terminal pro-endothelin-1 (CT-proET-1) and C-terminal pro-vasopressin (CT-proAVP or copeptin) immediately after birth in umbilical venous cord blood from 119 infants with a gestational age of 23-42 weeks and evaluated their possible functions.

Results: Cord blood levels of MR-proANP, MR- proADM, CT-proET-1, and CT-proAVP were considerably higher compared with normal adult levels. The CT-proAVP concentrations were 10-fold higher in term, and 70-fold higher in extremely preterm infants. MR-proANP showed 4-fold higher levels in term infants and 20-fold higher levels in extremely preterm infants. Levels of MR-proADM and CT-proET-1 were 2- to 3-fold higher in preterm and term infants. All four parameters showed significantly decreased values with increasing gestational age and a significant correlation between CT-proET-1 and MR-proADM.

Conclusion: Our results indicate that vasoactive and natriuretic mediators play a functionally relevant role in circulatory transition from fetal to neonatal life.

MeSH terms

  • Adrenomedullin / blood*
  • Adult
  • Atrial Natriuretic Factor / blood*
  • Endothelin-1 / blood*
  • Female
  • Fetal Blood / chemistry*
  • Gestational Age
  • Glycopeptides / blood*
  • Humans
  • Infant
  • Infant, Newborn
  • Infant, Premature / blood*
  • Male
  • Peptide Fragments / blood*
  • Protein Precursors / blood*
  • Retrospective Studies
  • Vasopressins / blood

Substances

  • C-terminal proendothelin-1
  • Endothelin-1
  • Glycopeptides
  • Peptide Fragments
  • Protein Precursors
  • copeptins
  • midregional pro-atrial natriuretic peptide, human
  • proAVP hormone
  • Vasopressins
  • Adrenomedullin
  • Atrial Natriuretic Factor