Clinical and molecular genetic factors affecting postoperative seizure control of 183 Chinese adult patients with low-grade gliomas

Eur J Neurol. 2012 Feb;19(2):298-306. doi: 10.1111/j.1468-1331.2011.03509.x. Epub 2011 Sep 6.

Abstract

Background and purpose: Seizures are a common symptom of patients with primary brain tumors, particularly low-grade gliomas (LGGs). Poor seizure control after surgery has a great adverse impact on quality of life in these patients. The present study aimed to identify clinical and molecular genetic factors that influence postoperative seizure control.

Methods: A series of 183 LGGs were analyzed by denaturing high-performance liquid chromatography (DHPLC) for 1p and 19q status and by immunohistochemical staining for expression of several molecular markers (P53, Ki-67, MMP-9 and MGMT), with particular emphasis on correlations with postoperative seizure control. Univariate and multivariate logistic regression analyses were used for statistic analysis.

Results: Of the 183 patients, 134 (73.2%) patients presented with seizures. Most of oligodendrogliomas and oligoastrocytomas had LOH 1p and LOH 19q, which were rarely seen in combination in astrocytomas (P<0.001). Oligodendroglial tumors were more likely to locate in frontal lobe (P=0.011) and present calcification on MRI (P=0.024). Temporal location (P=0.014), and high expression of mutated P53 (P=0.011) were associated with astrocytomas. Patients achieved much better seizure control after gross-total resection (P<0.001) than after subtotal resection. Patients without LOH 19q were more likely to have poor seizure control (P=0.004) than those with this alteration. Ki-67 was an independent molecular marker predicting poor seizure control (P=0.016) if over expressed.

Conclusions: Gross total resection of the tumor, LOH 19q and low Ki-67 expression were associated with favorable seizure control after surgery for the patients with LGGs. The possible involvement of other factors should be investigated further.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Asian People / genetics
  • Brain Neoplasms / complications
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / pathology
  • Brain Neoplasms / surgery
  • Chromosomes, Human, Pair 1
  • Chromosomes, Human, Pair 19
  • Female
  • Glioma / complications
  • Glioma / genetics*
  • Glioma / pathology
  • Glioma / surgery
  • Humans
  • Loss of Heterozygosity
  • Male
  • Middle Aged
  • Retrospective Studies
  • Seizures / etiology
  • Seizures / genetics*
  • Seizures / pathology
  • Seizures / surgery