In vitro anti Mycobacterium tuberculosis H37Rv activity of Lannea acida A. Rich from Burkina Faso

Pak J Biol Sci. 2011 Jan 1;14(1):47-52. doi: 10.3923/pjbs.2011.47.52.

Abstract

The cytotoxic and anti-Mycobacterium tuberculosis H37Rv activities of hydro-alcoholic extract of Lannea acida A. Rich (Anacardiaceae) were assessed. The cytoxicity evaluation was carried out on THP1 monocytoid cell line (after 24 h at 1; 5 and 10 microg mL(-1)) and showed only a slight modification of lactate dehydrogenase (LDH) release. The rate of monocytes in different stages of mitosis had been amended in absence and presence of extract as follows: Go/G1 58.83-59.83%; synthesis 21.95-18.64%; mitosis 16.67-15.77%; necrosis 2.65-5.64%. The percentage of inhibition of Mycobacterium tuberculosis proliferation was respectively 77.6 and 36.8% at 1.2 and 0.6 mg mL(-1) of extract. This is an interesting experimental study on antimicrobial and immune-stimulating properties of Lannea acida ethanol-water (70% v/v) extract which may contain potential antibacterial and immune-stimulating agents for clinical use.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anacardiaceae / metabolism*
  • Anti-Bacterial Agents / pharmacology
  • Burkina Faso
  • Cell Cycle
  • Cell Line
  • Ethanol / chemistry
  • Humans
  • In Vitro Techniques
  • L-Lactate Dehydrogenase / metabolism
  • Lipopolysaccharide Receptors / biosynthesis
  • Macrophages / microbiology
  • Mitosis
  • Monocytes / microbiology
  • Mycobacterium Infections / drug therapy
  • Mycobacterium Infections / epidemiology
  • Mycobacterium Infections / microbiology*
  • Mycobacterium tuberculosis / genetics*
  • Necrosis
  • Time Factors
  • Water / chemistry

Substances

  • Anti-Bacterial Agents
  • Lipopolysaccharide Receptors
  • Water
  • Ethanol
  • L-Lactate Dehydrogenase