Immunohistochemical study identifying prognostic biomolecular markers in nasopharyngeal carcinoma treated by radiotherapy

Head Neck. 2011 Oct;33(10):1458-66. doi: 10.1002/hed.21611. Epub 2010 Nov 4.

Abstract

Background: We evaluated the predictive significance of 14 reported markers using immunohistochemical study in nasopharyngeal carcinoma.

Methods: Immunohistochemical stainings were done in 38 patients for Met, cyclooxygenase-2 (COX-2), nm23-H1, epidermal growth factor receptor (EGFR), p63, early growth response factor 1 (Egr1), chromosome segregation 1-like (CSE1L), cathepsin-D (aspartyl protease), C-erbB2, p53, signal transducers and activators of transcription (STAT3/STAT5), CD138 (Syndecan-1), and LIN28 with the usual methods.

Results: The median follow-up time was 30 months (11-83 months). High Met and CD138 expression were statistically significant negative prognostic factors on survival. The expression of Egr1 had a positive prognostic effect on survival. The combined score of these 3 markers, Met plus CD138 minus Egr1, was a strong prognostic factor. The median survival curve was distinctly separated in accord with this combined score. No prognostic value was revealed in COX-2, nm23-H1, EGFR, p63, CSE1L, cathepsin-D, C-erbB2, p53, STAT3, STAT5, and LIN28.

Conclusions: The combined score of these markers could be used to stratify biomolecular risk groups.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Biomarkers, Tumor / metabolism*
  • Carcinoma / metabolism
  • Carcinoma / mortality
  • Carcinoma / therapy
  • Cathepsin D / metabolism
  • Cellular Apoptosis Susceptibility Protein / metabolism
  • Chemotherapy, Adjuvant
  • Cyclooxygenase 2 / metabolism
  • Disease-Free Survival
  • Early Growth Response Protein 1 / metabolism
  • ErbB Receptors / metabolism
  • Female
  • Follow-Up Studies
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • NM23 Nucleoside Diphosphate Kinases / metabolism
  • Nasopharyngeal Neoplasms / metabolism*
  • Nasopharyngeal Neoplasms / mortality*
  • Nasopharyngeal Neoplasms / therapy
  • Prognosis
  • Proto-Oncogene Proteins c-met / metabolism
  • RNA-Binding Proteins / metabolism
  • Radiotherapy, Intensity-Modulated
  • Receptor, ErbB-2 / metabolism
  • STAT3 Transcription Factor / metabolism
  • STAT5 Transcription Factor / metabolism
  • Syndecan-1 / metabolism
  • Transcription Factors / metabolism
  • Tumor Suppressor Protein p53 / metabolism
  • Tumor Suppressor Proteins / metabolism

Substances

  • Biomarkers, Tumor
  • Cellular Apoptosis Susceptibility Protein
  • Early Growth Response Protein 1
  • Lin28A protein, human
  • NM23 Nucleoside Diphosphate Kinases
  • RNA-Binding Proteins
  • STAT3 Transcription Factor
  • STAT5 Transcription Factor
  • Syndecan-1
  • TP63 protein, human
  • Transcription Factors
  • Tumor Suppressor Protein p53
  • Tumor Suppressor Proteins
  • Cyclooxygenase 2
  • ErbB Receptors
  • MET protein, human
  • Proto-Oncogene Proteins c-met
  • Receptor, ErbB-2
  • NME1 protein, human
  • Cathepsin D