Oncostatin M and IL-6 induce u-PA and VEGF in prostate cancer cells and correlate in vivo

Anticancer Res. 2011 Oct;31(10):3273-8.

Abstract

Background/aim: Oncostatin M (OSM) and interleukin-6 (IL-6) are growth factors for prostate cancer (PC). Vascular endothelial growth factor (VEGF) and urokinase-type plasminogen-activator (u-PA) have been implicated in tumour progression. A possible interaction between IL-6, OSM, u-PA and VEGF in PC was investigated.

Materials and methods: Primary prostate epithelial cells (PPEC) and DU-145 PC cells were treated with IL-6 or OSM and the effects on u-PA and VEGF expression were studied. Plasma levels of IL-6, OSM, u-PA and VEGF were determined in patients with or without PC.

Results: In DU-145 cells, OSM and IL-6 up-regulated u-PA and VEGF significantly. Higher levels of IL-6 and OSM in metastasising PC than in nonmetastasising PC and benign prostatic hyperplasia (BPH) and correlations between IL-6, OSM, u-PA and VEGF were found.

Conclusion: OSM and IL-6 increase u-PA and VEGF in DU-145 cells but not in PPEC and possibly, by promoting matrix degradation and angiogenesis, could play a role in the pathogenesis of prostate cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Cell Line, Tumor
  • Cytokine Receptor gp130 / metabolism
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Interleukin-6 / blood
  • Interleukin-6 / pharmacology*
  • Ligands
  • Male
  • Neoplasm Metastasis
  • Oncostatin M / blood
  • Oncostatin M / pharmacology*
  • Prostate / drug effects
  • Prostate / pathology
  • Prostatic Hyperplasia / blood
  • Prostatic Hyperplasia / pathology
  • Prostatic Neoplasms / blood
  • Prostatic Neoplasms / genetics
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / pathology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Urokinase-Type Plasminogen Activator / blood
  • Urokinase-Type Plasminogen Activator / genetics
  • Urokinase-Type Plasminogen Activator / metabolism*
  • Vascular Endothelial Growth Factor A / blood
  • Vascular Endothelial Growth Factor A / genetics
  • Vascular Endothelial Growth Factor A / metabolism*

Substances

  • IL6 protein, human
  • Interleukin-6
  • Ligands
  • RNA, Messenger
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A
  • Oncostatin M
  • Cytokine Receptor gp130
  • Urokinase-Type Plasminogen Activator