Methicillin-susceptible Staphylococcus aureus clone related to the early pandemic phage type 80/81 causing an outbreak among residents of three occupational centres in Barcelona, Spain

Clin Microbiol Infect. 2012 Jul;18(7):662-7. doi: 10.1111/j.1469-0691.2011.03663.x. Epub 2011 Oct 4.

Abstract

In the 1950s an unusually virulent and transmissible penicillin-resistant Staphylococcus aureus clone harbouring Panton-Valentine leukocidin (PVL) genes, known as phage type 80/81 and subsequently identified as multilocus sequence type (ST) 30, emerged and caused serious infections in hospitals and the community. We describe an outbreak of skin infections caused by a PVL-positive, methicillin-susceptible S. aureus (MSSA) strain of ST1472, related to phage type 80/81, in three associated occupational centres. After identification of the first patient an active case-finding strategy was initiated among the three centres. Epidemiological and clinical features were indistinguishable from outbreaks currently caused by community-acquired methicillin-resistant S. aureus. The S. aureus was cultured and identified from nasal swabs and skin lesions by conventional methods; PVL was detected using a PCR assay. Pulsed-field gel electrophoresis and DNA-array-based genotyping were applied to MSSA isolates. MSSA was identified in nasal swabs from 49 of 133 individuals (37%). A single pulsed-field gel electrophoresis pattern, belonging to ST1472 (CC30) and PVL positivity, were detected in 20 individuals, including eight of 18 skin cultures, i.e. 15% of the screened individuals were colonized by the epidemic strain. Nasal and cutaneous decontamination with 5% nasal mupirocin ointment and 2% aqueous chlorhexidine was implemented for all individuals. Patients with active skin infections were treated with a first-generation cephalosporin. General recommendations were made to prevent cross-transmission. No new cases were reported over the following 90 days.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Bacterial Toxins / genetics
  • Bacteriophage Typing*
  • Community-Acquired Infections / epidemiology
  • Community-Acquired Infections / microbiology
  • Disease Outbreaks*
  • Electrophoresis, Gel, Pulsed-Field
  • Exotoxins / genetics
  • Female
  • Genotype
  • Humans
  • Infection Control / methods
  • Leukocidins / genetics
  • Male
  • Methicillin
  • Microarray Analysis
  • Middle Aged
  • Molecular Typing
  • Nose / microbiology
  • Polymerase Chain Reaction
  • Skin / microbiology
  • Spain / epidemiology
  • Staphylococcal Skin Infections / epidemiology*
  • Staphylococcal Skin Infections / microbiology*
  • Staphylococcus aureus / classification*
  • Staphylococcus aureus / isolation & purification
  • Young Adult

Substances

  • Bacterial Toxins
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin
  • Methicillin