A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin

Protein Sci. 2011 Dec;20(12):2095-101. doi: 10.1002/pro.749. Epub 2011 Nov 9.

Abstract

11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Crystallography, X-Ray
  • Dansyl Compounds / chemistry
  • Dansyl Compounds / metabolism*
  • Fatty Acids / chemistry
  • Fatty Acids / metabolism*
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Humans
  • Models, Molecular
  • Myristates / chemistry
  • Myristates / metabolism*
  • Protein Binding
  • Serum Albumin / chemistry
  • Serum Albumin / metabolism*

Substances

  • Dansyl Compounds
  • Fatty Acids
  • Fluorescent Dyes
  • Myristates
  • Serum Albumin
  • 11-(dansylamino)undecanoic acid