Physical properties such as surface hardness of dental materials are directly linked to their clinical behavior. The aim of this study was to investigate the influence of extended curing time on the polymerization depth offluoride-containing materials used as pit and fissure sealants. Conventional and extended exposure times (20 and 60 seconds) were used to photoactivate a gold-standard pit and fissure sealant (Fluroshield, Dentsply) and a flowable composite (PermaFlo, Ultradent). Twenty square-shaped samples (n=5) were prepared using a LED device (Bluephase 16i, Ivoclar). The Knoop Hardness Number (KHN) was calculated for the top and bottom surface of each sample 24 hours after polymerization. Bottom/top hardness ratio (B/T KHN) was than calculated. Averages were analyzed by two-way ANOVA and Tukey test (alpha=0.05). The flowable composite had higher KHN than conventional pit and fissure sealant for all experimental conditions (p<0.05). The 60-second photoactivation time increased KHN at the bottom surface and B/T KHN only of composite specimens. The flowable composite had better physical properties than the pit and fissure sealant, and they were improved by extended curing time.