Tumor grafts derived from women with breast cancer authentically reflect tumor pathology, growth, metastasis and disease outcomes

Nat Med. 2011 Oct 23;17(11):1514-20. doi: 10.1038/nm.2454.

Abstract

Development and preclinical testing of new cancer therapies is limited by the scarcity of in vivo models that authentically reproduce tumor growth and metastatic progression. We report new models for breast tumor growth and metastasis in the form of transplantable tumors derived directly from individuals undergoing treatment for breast cancer. These tumor grafts illustrate the diversity of human breast cancer and maintain essential features of the original tumors, including metastasis to specific sites. Co-engraftment of primary human mesenchymal stem cells maintains phenotypic stability of the grafts and increases tumor growth by promoting angiogenesis. We also report that tumor engraftment is a prognostic indicator of disease outcome for women with newly diagnosed breast cancer; orthotopic breast tumor grafting is a step toward individualized models for tumor growth, metastasis and prognosis. This bank of tumor grafts also serves as a publicly available resource for new models in which to study the biology of breast cancer.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Breast Neoplasms / pathology*
  • Breast Neoplasms / physiopathology*
  • Breast Neoplasms / therapy
  • Disease Progression*
  • Female
  • Gene Expression Profiling
  • Humans
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / physiology
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Microarray Analysis
  • Neoplasm Metastasis / pathology*
  • Neoplasm Metastasis / physiopathology*
  • Neoplasm Transplantation / methods*
  • Neovascularization, Pathologic
  • Survival Rate
  • Transplantation, Heterologous / methods*

Associated data

  • GEO/GSE32532