Chemical structure of wall teichoic acid isolated from Enterococcus faecium strain U0317

Carbohydr Res. 2011 Dec 13;346(17):2816-9. doi: 10.1016/j.carres.2011.09.026. Epub 2011 Oct 2.

Abstract

Wall teichoic acid (WTA) was isolated from Enterococcus faecium strain U0317 and structurally characterized using (1)H, (13)C, and (31)P NMR spectroscopy, including two-dimensional COSY, TOCSY, ROESY, HMQC, and HMBC experiments. Further compositional determination was undertaken using classical chemical methods and HF treatment followed by GLC and GLC-MS analyses. The repeating unit of WTA consisted of two residues of 2-acetamido-2-deoxy-D-galactose, glycerol (Gro), and phosphate, and has the structure shown below: [See formula in text].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cell Wall / chemistry*
  • Enterococcus faecium*
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / isolation & purification
  • Magnetic Resonance Spectroscopy
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Teichoic Acids / chemistry*
  • Teichoic Acids / isolation & purification

Substances

  • Lipopolysaccharides
  • Teichoic Acids
  • lipoteichoic acid