Abstract
Using a superoxide (O(2)(-)) generation assay system with differentiated HL-60 cells, 1,2-di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol (DLGG) was identified as an O(2)(-) generation inhibitor from Perilla frutescens var. crispa (a local variety, kida-chirimen shiso). DLGG suppressed the O(2)(-) level in a dose-dependent manner with an IC(50) value of 21 µM, comparable to those of rosmarinic acid (RoA, IC(50) = 29 µM) and caffeic acid (CA, IC(50) = 30 µM). While RoA and CA also dose-dependently inhibited O(2)(-) generation in a xanthine-xanthine oxidase system, DLGG had no effect in the same system. Thus DLGG appeared to decrease the O(2)(-) level in the HL-60 assay system by mechanisms different from those of RoA and CA, which appeared to act as O(2)(-) scavengers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Caffeic Acids / metabolism
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Cinnamates / metabolism
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Depsides / metabolism
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Free Radical Scavengers / chemistry
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology*
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Glucosides / chemistry
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Glucosides / isolation & purification
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Glucosides / pharmacology*
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Glycerol / analogs & derivatives*
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Glycerol / chemistry
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Glycerol / isolation & purification
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Glycerol / pharmacology
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HL-60 Cells
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Humans
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Inhibitory Concentration 50
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Molecular Structure
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Perilla frutescens / chemistry*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Rosmarinic Acid
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Superoxides / antagonists & inhibitors*
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Xanthine Oxidase / metabolism
Substances
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1,2-dilinolenoyl-3-galactopyranosylglycerol
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Caffeic Acids
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Cinnamates
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Depsides
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Free Radical Scavengers
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Glucosides
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Plant Extracts
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Superoxides
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Xanthine Oxidase
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Glycerol
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caffeic acid