1,2-Di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol as a superoxide generation inhibitor from Perilla frutescens var. crispa

Biosci Biotechnol Biochem. 2011;75(11):2240-2. doi: 10.1271/bbb.110414. Epub 2011 Nov 7.

Abstract

Using a superoxide (O(2)(-)) generation assay system with differentiated HL-60 cells, 1,2-di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol (DLGG) was identified as an O(2)(-) generation inhibitor from Perilla frutescens var. crispa (a local variety, kida-chirimen shiso). DLGG suppressed the O(2)(-) level in a dose-dependent manner with an IC(50) value of 21 µM, comparable to those of rosmarinic acid (RoA, IC(50) = 29 µM) and caffeic acid (CA, IC(50) = 30 µM). While RoA and CA also dose-dependently inhibited O(2)(-) generation in a xanthine-xanthine oxidase system, DLGG had no effect in the same system. Thus DLGG appeared to decrease the O(2)(-) level in the HL-60 assay system by mechanisms different from those of RoA and CA, which appeared to act as O(2)(-) scavengers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caffeic Acids / metabolism
  • Cinnamates / metabolism
  • Depsides / metabolism
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / isolation & purification
  • Free Radical Scavengers / pharmacology*
  • Glucosides / chemistry
  • Glucosides / isolation & purification
  • Glucosides / pharmacology*
  • Glycerol / analogs & derivatives*
  • Glycerol / chemistry
  • Glycerol / isolation & purification
  • Glycerol / pharmacology
  • HL-60 Cells
  • Humans
  • Inhibitory Concentration 50
  • Molecular Structure
  • Perilla frutescens / chemistry*
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Rosmarinic Acid
  • Superoxides / antagonists & inhibitors*
  • Xanthine Oxidase / metabolism

Substances

  • 1,2-dilinolenoyl-3-galactopyranosylglycerol
  • Caffeic Acids
  • Cinnamates
  • Depsides
  • Free Radical Scavengers
  • Glucosides
  • Plant Extracts
  • Superoxides
  • Xanthine Oxidase
  • Glycerol
  • caffeic acid