Site-selective modification of proteins for the synthesis of structurally defined multivalent scaffolds

Chem Commun (Camb). 2012 Jan 14;48(4):522-4. doi: 10.1039/c1cc16039g. Epub 2011 Nov 9.

Abstract

A combination of classical site-directed mutagenesis, genetic code engineering and bioorthogonal reactions delivered a chemically modified barstar protein with one or four carbohydrates installed at specific residues. These protein conjugates were employed in multivalent binding studies, which support the use of proteins as structurally defined scaffolds for the presentation of multivalent ligands.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / chemistry*
  • Bacillus / genetics*
  • Bacillus / metabolism
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Carbohydrates / chemistry*
  • Ligands
  • Models, Molecular
  • Mutagenesis, Site-Directed / methods
  • Protein Binding
  • Protein Engineering / methods*

Substances

  • Bacterial Proteins
  • Carbohydrates
  • Ligands
  • barstar protein, Bacillus amyloliquefaciens