In vivo evaluation in cynomolgus monkey brain and metabolism of [¹⁸F]fluorodeprenyl: a new MAO-B pet radioligand

Synapse. 2012 Apr;66(4):323-30. doi: 10.1002/syn.21514. Epub 2011 Dec 30.

Abstract

In this study, we evaluated the in vivo characteristics of a new monoamine oxidase type B (MAO-B) radioligand, [¹⁸F]fluorodeprenyl, by positron emission tomography (PET) in two cynomolgus monkeys. The brain uptake of [¹⁸F]fluorodeprenyl was more than 7% (600% SUV) of the total injected radioactivity and similar to that of [¹¹C]deprenyl, an established MAO-B radioligand. The highest uptake was observed in the striatum, one of the MAO-B-rich regions, with a peak at approximately 2-3 min after injection, followed by lower uptake in the thalamus and the cortex and lowest uptake in the cerebellum. Brain uptake of [¹⁸F]fluorodeprenyl was largely inhibited by preadministration of the MAO-B inhibitor, L-deprenyl, whereas clorgyline, a MAO Type A blocker, had no significant inhibitory effect, thus demonstrating selectivity for MAO-B. [¹⁸F]Fluorodeprenyl showed relatively slow metabolism with the presence of two radiometabolite peaks with similar retention time as the labeled metabolites of [¹¹C]deprenyl. These results suggest that [¹⁸F]fluorodeprenyl is a potential PET radioligand for visualization of MAO-B activity.

MeSH terms

  • Animals
  • Brain / diagnostic imaging*
  • Brain / metabolism
  • Chromatography, High Pressure Liquid
  • Fluorine Radioisotopes / metabolism*
  • Macaca fascicularis
  • Monoamine Oxidase / metabolism*
  • Positron-Emission Tomography / methods*
  • Radiopharmaceuticals / metabolism*
  • Selegiline / chemistry
  • Selegiline / metabolism*

Substances

  • Fluorine Radioisotopes
  • Radiopharmaceuticals
  • Selegiline
  • Monoamine Oxidase