Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling

Xiaolei Zuo; Fan Xia; Adriana Patterson; H Tom Soh; Yi Xiao; Kevin W Plaxco

doi:10.1002/cbic.201100592

Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling

Chembiochem. 2011 Dec 16;12(18):2745-7. doi: 10.1002/cbic.201100592. Epub 2011 Nov 28.

Authors

Xiaolei Zuo¹, Fan Xia, Adriana Patterson, H Tom Soh, Yi Xiao, Kevin W Plaxco

Affiliation

¹ Department of Chemistry and Biochemistry, University of California, Santa Barbara, USA.

PMID: 22125097
DOI: 10.1002/cbic.201100592

Abstract

We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast cancer cells in a single-measurement fluorescence assay that avoids the problematic PCR and gel analysis of the current "gold-standard" assay.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Cell Line
Chromatography, High Pressure Liquid
DNA Probes
Exodeoxyribonucleases / metabolism*
Humans
Mass Spectrometry
Polymerase Chain Reaction / methods*
Telomerase / metabolism*

Substances

DNA Probes
Telomerase
Exodeoxyribonucleases
exodeoxyribonuclease III