Protective effect of Moringa oleifera leaves against gentamicin-induced nephrotoxicity in rabbits

Exp Toxicol Pathol. 2013 Mar;65(3):335-9. doi: 10.1016/j.etp.2011.11.006. Epub 2011 Dec 23.

Abstract

Oxidative stress due to abnormal production of reactive oxygen species has been implicated in the nephrotoxicity induced by gentamicin. The nephroprotective effect of aqueous-ethanolic extract of Moringa oleifera leaves (150 and 300 mg/kg) was evaluated against gentamicin-induced (80 mg/kg) renal injury in rabbits. Serum urea and creatinine levels were evaluated as the markers of renal nephrotoxicity. At the end of the experiment, the kidneys of rabbits were excised for histological examinations and determination of lipid peroxidation levels. Serum urea and creatinine levels were reduced in the M. oleifera (150 and 300 mg/kg) plus gentamicin treated groups. On histological examinations, kidney of intoxicated rabbits groups which received M. oleifera extract showed reparative tendencies. A highly significant (p < 0.01) elevation was observed in lipid peroxidation (LPO) level in the kidneys of gentamicin-intoxicated rabbits whereas combined treatment of M. oleifera and gentamicin group showed a highly significant (p < 0.01) depletion in LPO. The present study indicates that aqueous-ethanolic extract of M. oleifera leaves attenuates renal injury in rabbits treated with gentamicin, possibly by inhibiting lipid peroxidation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / toxicity*
  • Antioxidants / isolation & purification
  • Antioxidants / therapeutic use*
  • Gentamicins / toxicity*
  • Kidney / drug effects*
  • Kidney / metabolism
  • Kidney / pathology
  • Kidney Diseases / chemically induced
  • Kidney Diseases / metabolism
  • Kidney Diseases / pathology
  • Kidney Diseases / prevention & control*
  • Kidney Function Tests
  • Lipid Peroxidation / drug effects
  • Male
  • Moringa oleifera / chemistry*
  • Oxidative Stress / drug effects
  • Plant Extracts / isolation & purification
  • Plant Extracts / therapeutic use*
  • Plant Leaves / chemistry
  • Rabbits

Substances

  • Anti-Bacterial Agents
  • Antioxidants
  • Gentamicins
  • Plant Extracts