Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin-Arg-lysozyme complex

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1;68(Pt 2):154-8. doi: 10.1107/S1744309111056132. Epub 2012 Jan 25.

Abstract

Crystallization of contaminating proteins is a frequently encountered problem for macromolecular crystallographers. In this study, an attempt was made to obtain a binary cocrystal structure of the SH3 domain of cortactin and a 17-residue peptide from the Arg nonreceptor tyrosine kinase encompassing a PxxPxxPxxP (PxxP1) motif. However, cocrystals could only be obtained in the presence of trace amounts of a contaminating protein. A structure solution obtained by molecular replacement followed by ARP/wARP automatic model building allowed a 'sequence-by-crystallography' approach to discover that the contaminating protein was lysozyme. This 1.65 Å resolution crystal structure determination of a 1:1:1 heterotrimeric complex of Arg, cortactin and lysozyme thus provides an unusual `caveat emptor' warning of the dangers that underpurified proteins harbor for macromolecular crystallographers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginine / chemistry
  • Cortactin / chemistry*
  • Cortactin / metabolism
  • Crystallization
  • Crystallography, X-Ray
  • Mice
  • Models, Molecular
  • Muramidase / chemistry*
  • Muramidase / isolation & purification
  • Muramidase / metabolism
  • Protein Binding
  • Protein Structure, Quaternary
  • Protein Structure, Tertiary

Substances

  • Cortactin
  • Arginine
  • Muramidase

Associated data

  • PDB/3ULR