Identification of peptide features in precursor spectra using Hardklör and Krönik

Curr Protoc Bioinformatics. 2012 Mar:Chapter 13:Unit13.18. doi: 10.1002/0471250953.bi1318s37.

Abstract

Hardklör and Krönik are software tools for feature detection and data reduction of high-resolution mass spectra. Hardklör is used to reduce peptide isotope distributions to a single monoisotopic mass and charge state, and can deconvolve overlapping peptide isotope distributions. Krönik filters, validates, and summarizes peptide features identified with Hardklör from data obtained during liquid chromatography mass spectrometry (LC-MS). Both software tools contain a simple user interface and can be run from nearly any desktop computer. These tools are freely available from http://proteome.gs.washington.edu/software/hardklor.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Chromatography, Liquid / methods
  • Mass Spectrometry / methods
  • Peptides / chemistry*
  • Proteomics
  • Software*

Substances

  • Peptides