Evaluation of a rapid immunochromatographic ODK-0901 test for detection of pneumococcal antigen in middle ear fluids and nasopharyngeal secretions

PLoS One. 2012;7(3):e33620. doi: 10.1371/journal.pone.0033620. Epub 2012 Mar 20.

Abstract

Since the incidence of penicillin-resistant Streptococcus pneumoniae has been increasing at an astonishing rate throughout the world, the need for accurate and rapid identification of pneumococci has become increasingly important to determine the appropriate antimicrobial treatment. We have evaluated an immunochromatographic test (ODK-0901) that detects pneumococcal antigens using 264 middle ear fluids (MEFs) and 268 nasopharyngeal secretions (NPSs). A sample was defined to contain S. pneumoniae when optochin and bile sensitive alpha hemolytic streptococcal colonies were isolated by culture. The sensitivity and specificity of the ODK-0901 test were 81.4% and 80.5%, respectively, for MEFs from patients with acute otitis media (AOM). In addition, the sensitivity and specificity were 75.2% and 88.8%, respectively, for NPSs from patients with acute rhinosinusitis. The ODK-0901 test may provide a rapid and highly sensitive evaluation of the presence of S. pneumoniae and thus may be a promising method of identifying pneumococci in MEFs and NPSs.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Adolescent
  • Adult
  • Bacterial Proteins / genetics
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Chromatography, Affinity*
  • Ear, Middle / microbiology
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Middle Aged
  • Nasopharynx / metabolism*
  • Otitis Media with Effusion / diagnosis*
  • Otitis Media with Effusion / microbiology
  • Pneumococcal Infections / diagnosis*
  • Pneumococcal Infections / microbiology
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Rhinitis / diagnosis*
  • Rhinitis / microbiology
  • Sensitivity and Specificity
  • Sinusitis / diagnosis*
  • Sinusitis / microbiology
  • Streptococcus pneumoniae / immunology
  • Streptococcus pneumoniae / isolation & purification*
  • Teichoic Acids / immunology
  • Teichoic Acids / metabolism*
  • Young Adult

Substances

  • Bacterial Proteins
  • RNA, Messenger
  • Teichoic Acids
  • pneumococcal surface protein A