Distribution of P75 neurotrophin receptor in adult human cochlea--an immunohistochemical study

Cell Tissue Res. 2012 Jun;348(3):407-15. doi: 10.1007/s00441-012-1395-7. Epub 2012 Mar 31.

Abstract

Mechanisms underlying the unique survival property of human spiral neurons are yet to be explored. P75 (p75(NTR)) is a low affinity receptor for neurotrophins and is known to interact with Trk receptors to modulate ligand binding and signaling. Up-regulation of this receptor was found to be associated with apoptosis as well as with cell proliferation. Its distribution and injury-induced change in expression pattern in the cochlea have been mainly studied in rodents. There is still no report concerning p75(NTR) in post-natal human inner ear. We analyzed, for the first time, p75(NTR) expression in five freshly fixed human cochleae by using immunohistochemistry techniques, including myelin basic protein (MBP) as a myelin sheath marker and TrkB as the human spiral neuron marker, and by using thin optical sectioning of laser confocal microscopy. The inner ear specimens were obtained from adult patients who had normal pure tone thresholds before the surgical procedures, via a trans-cochlear approach for removal of giant posterior cranial fossa meningioma. The expression of p75(NTR) was investigated and localized in the glial cells, including Schwann cells and satellite glial cells in the Rosenthal canal, in the central nerve bundles within the modiolus, and in the osseous spiral lamina of the human cochleae. The biological significance of p75(NTR) in human cochlea is discussed.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibody Specificity / immunology
  • Cochlea / cytology
  • Cochlea / metabolism*
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Myelin Basic Protein / metabolism
  • Nerve Tissue Proteins / immunology
  • Nerve Tissue Proteins / metabolism*
  • Organ of Corti / cytology
  • Organ of Corti / metabolism
  • Receptors, Nerve Growth Factor / immunology
  • Receptors, Nerve Growth Factor / metabolism*

Substances

  • Myelin Basic Protein
  • NGFR protein, human
  • Nerve Tissue Proteins
  • Receptors, Nerve Growth Factor