Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide

Biochem Biophys Res Commun. 2012 May 4;421(2):208-13. doi: 10.1016/j.bbrc.2012.03.136. Epub 2012 Apr 4.

Abstract

The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion Molecules / chemistry*
  • Crystallization / methods
  • Crystallography, X-Ray
  • Deuterium Oxide / chemistry*
  • Fimbriae Proteins / chemistry*
  • Fimbriae, Bacterial / chemistry*
  • Protein Multimerization
  • Protein Structure, Tertiary
  • Salmonella enteritidis / metabolism*

Substances

  • Cell Adhesion Molecules
  • SefD protein, Salmonella
  • Fimbriae Proteins
  • Deuterium Oxide