Abstract
In this article, the first isolation of Mycobacterium kyorinense specimens in Brazil is described. M. kyorinense is a recently identified species, with a few strains reported only in Japan. The Brazilian isolates were initially identified as Mycobacterium celatum by PCR restriction enzyme pattern analysis (PRA) with hsp65. However, biochemical tests indicated the same profile of M. kyorinense and distinguished them from M. celatum and Mycobacterium branderi. The sequencing of the hsp65, rpoB, and 16S rRNA genes allowed the accurate identification of isolates as M. kyorinense.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Brazil
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Chaperonin 60 / genetics
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA-Directed RNA Polymerases / genetics
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Humans
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Molecular Sequence Data
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Mycobacterium / classification*
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Mycobacterium / isolation & purification*
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Mycobacterium Infections / diagnosis*
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Mycobacterium Infections / microbiology*
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Phylogeny
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
Substances
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Bacterial Proteins
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Chaperonin 60
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DNA, Bacterial
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RNA, Ribosomal, 16S
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heat-shock protein 65, Mycobacterium
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DNA-Directed RNA Polymerases
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RNA polymerase beta subunit
Associated data
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GENBANK/JN634643
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GENBANK/JN974461
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GENBANK/JQ744020