Biochemical methods for quantifying sphingolipids: ceramide, sphingosine, sphingosine kinase-1 activity, and sphingosine-1-phosphate

Methods Mol Biol. 2012:874:1-20. doi: 10.1007/978-1-61779-800-9_1.

Abstract

Sphingolipids (ceramide, sphingosine, and sphingosine-1-phosphate) are bioactive lipids with important biological functions in proliferation, apoptosis, angiogenesis, and inflammation. Herein, we describe easy and rapid biochemical methods with the use of radiolabeled molecules ((3)H, (32)P) for their mass determination. Quantitation of sphingosine kinase-1 activity, the most studied isoform, is also included.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Extracts
  • Cell Line, Tumor
  • Ceramides / analysis*
  • Humans
  • Lysophospholipids / analysis*
  • Phosphotransferases (Alcohol Group Acceptor) / analysis*
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Spectrophotometry
  • Sphingosine / analogs & derivatives*
  • Sphingosine / analysis*

Substances

  • Cell Extracts
  • Ceramides
  • Lysophospholipids
  • sphingosine 1-phosphate
  • Phosphotransferases (Alcohol Group Acceptor)
  • sphingosine kinase
  • Sphingosine