High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in Staphylococcus epidermidis

J Microbiol Methods. 2012 Aug;90(2):134-6. doi: 10.1016/j.mimet.2012.04.002. Epub 2012 Apr 25.

Abstract

We describe a novel HRM-PCR (high-resolution melting) assay capable of the accurate identification of the G2576T point mutation in domain V of the 23S rRNA genes attributed to linezolid resistance in Staphylococcus epidermidis. This rapid method demonstrated 100% correlation with the previously established NheI restriction digest assay.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Acetamides / pharmacology*
  • Anti-Bacterial Agents / pharmacology*
  • DNA, Bacterial / genetics*
  • DNA, Ribosomal / genetics
  • Drug Resistance, Bacterial*
  • Humans
  • Linezolid
  • Microbial Sensitivity Tests / methods
  • Oxazolidinones / pharmacology*
  • Point Mutation
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 23S / genetics
  • Sensitivity and Specificity
  • Staphylococcus epidermidis / drug effects*
  • Staphylococcus epidermidis / genetics*
  • Transition Temperature*

Substances

  • Acetamides
  • Anti-Bacterial Agents
  • DNA, Bacterial
  • DNA, Ribosomal
  • Oxazolidinones
  • RNA, Bacterial
  • RNA, Ribosomal, 23S
  • Linezolid