Intrinsic features of Aedes aegypti genes affect transcriptional responsiveness of mosquito genes to dengue virus infection

Infect Genet Evol. 2012 Oct;12(7):1413-8. doi: 10.1016/j.meegid.2012.04.027. Epub 2012 May 3.

Abstract

Dengue virus infection causes significant morbidity and mortality in humans world-wide. The Aedes aegypti mosquito is the major vector that spreads dengue virus to humans. Interaction between dengue viruses and A. aegypti is a multi-factorial phenomena that is determined by both virus and mosquito genotypes. Although, studies have suggested significant association of mosquito vectorial capacity with population variation of dengue virus, specifications of the vector factors that may influence vector-virus compatibility are very limited in the literature. Recently, we have shown that a large number of genes are differentially expressed between MOYO-S (susceptible) and MOYO-R (refractory) A. aegypti strains upon infection with dengue virus (JAM-1409 genotype). In the current study, we show that specific intrinsic features of A. aegypti genes are significantly associated with 'responsiveness' of mosquito genes to dengue infection. Binomial logistic regression analysis further reveals differential marginal effects of these features on gene responsiveness of mosquitoes to the viral infection. Thus, our result shows that intrinsic features of genes significantly affect differential expression of A. aegypti genes to dengue infection. The information will benefit further investigations on evolution of genes among natural populations of A. aegypti conferring differential susceptibility to dengue virus.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aedes / genetics*
  • Aedes / virology
  • Animals
  • Dengue Virus / physiology*
  • Gene Expression Regulation*
  • Genes, Insect*
  • Host-Pathogen Interactions / genetics
  • Insect Vectors / genetics*
  • Insect Vectors / virology
  • Logistic Models
  • Oligonucleotide Array Sequence Analysis
  • Transcription, Genetic
  • Transcriptome