Increased expression of TrkB and Capzb2 accompanies preserved cognitive status in early Alzheimer disease pathology

J Neuropathol Exp Neurol. 2012 Jul;71(7):654-64. doi: 10.1097/NEN.0b013e31825d06b7.

Abstract

Brain-derived neurotrophic factor (BDNF) and its receptor tyrosine kinase B (TrkB) may influence brain reserve, the ability of the brain to tolerate pathological changes without significant decline in function. Here, we explore whether a specifically vulnerable population of human neurons shows a compensatory response to the neuropathological changes of Alzheimer disease (AD) and whether that response depends on an upregulation of the BDNF pathway. We observed increased neuronal TrkB expression associated with early-stage AD pathology (Braak and Braak stages I-II) in hippocampal CA1 region samples from cognitively intact Framingham Heart Study subjects (n = 5) when compared with cognitively intact individuals with no neurofibrillary tangles (n = 4). Because BDNF/TrkB signaling affects memory formation and retention through modification of the actin cytoskeleton, we examined the expression of actin capping protein β2 (Capzb2), a marker of actin cytoskeleton reorganization. Capzb2 expression was also significantly increased in CA1 hippocampal neurons of cognitively intact subjects with early-stage AD pathology. Our data suggest that increased expression of TrkB and Capzb2 accompanies adequate brain reserve in the initial stages of AD pathology. In subsequent stages of AD, the higher levels of TrkB and Capzb2 expression achieved may not be sufficient to prevent cognitive decline.

Publication types

  • Multicenter Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Capping Proteins / genetics
  • Actin Capping Proteins / metabolism*
  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / complications*
  • Alzheimer Disease / pathology*
  • Brain-Derived Neurotrophic Factor / genetics
  • Brain-Derived Neurotrophic Factor / metabolism
  • Cognition Disorders / diagnosis
  • Cognition Disorders / etiology*
  • Dementia / complications
  • Female
  • Hippocampus / metabolism*
  • Humans
  • Laser Capture Microdissection
  • Male
  • Neuropsychological Tests
  • RNA, Messenger / metabolism
  • Receptor, trkB / genetics
  • Receptor, trkB / metabolism*
  • Signal Transduction / physiology
  • Silver Staining
  • Up-Regulation / physiology

Substances

  • Actin Capping Proteins
  • Brain-Derived Neurotrophic Factor
  • RNA, Messenger
  • Receptor, trkB