Assessment of whole-genome mapping in a well-defined outbreak of Salmonella enterica serotype Saintpaul

P D Fey; P C Iwen; E B Zentz; A M Briska; J K Henkhaus; K A Bryant; M A Larson; R K Noel; S H Hinrichs

doi:10.1128/JCM.01320-12

Assessment of whole-genome mapping in a well-defined outbreak of Salmonella enterica serotype Saintpaul

J Clin Microbiol. 2012 Sep;50(9):3063-5. doi: 10.1128/JCM.01320-12. Epub 2012 Jun 20.

Authors

P D Fey¹, P C Iwen, E B Zentz, A M Briska, J K Henkhaus, K A Bryant, M A Larson, R K Noel, S H Hinrichs

Affiliation

¹ Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA. pfey@unmc.edu

Abstract

We investigated the use of whole-genome mapping and pulsed-field gel electrophoresis (PFGE) with isolates from an outbreak of Salmonella enterica serotype Saintpaul. PFGE and whole-genome mapping were concordant with 22 of 23 isolates. Whole-genome mapping is a viable alternative tool for the epidemiological analysis of Salmonella food-borne disease investigations.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Chromosome Mapping / methods*
Disease Outbreaks*
Electrophoresis, Gel, Pulsed-Field / methods
Humans
Molecular Epidemiology / methods
Molecular Typing / methods*
Salmonella Infections / epidemiology*
Salmonella Infections / microbiology*
Salmonella enterica / classification*
Salmonella enterica / genetics*
Salmonella enterica / isolation & purification