Activin A and its regulatory molecules in placenta and fetal membranes of women with preterm premature rupture of the membranes associated with acute chorioamnionitis

Am J Reprod Immunol. 2012 Nov;68(5):392-9. doi: 10.1111/j.1600-0897.2012.01180.x. Epub 2012 Jul 30.

Abstract

LABELED PROBLEM: To investigate regulation of activin A and related molecules in placenta/fetal membranes from preterm premature rupture of membranes (pPROM) associated with acute chorioamnionitis (ACA).

Method of study: Tissues were obtained from women with spontaneous preterm deliveries (PTD), pPROM without ACA, pPROM with ACA. Activin A, follistatin, and nodal and cripto mRNA were measured by RT-PCR.

Results: Activin A mRNA was up-regulated in tissues from pPROM, in presence or absence of HCA, respect to PTD and in pPROM with ACA respect to pPROM without ACA. Follistatin mRNA expression did not differ between the groups. In placenta, nodal mRNA showed the same trend of activin A, while cripto was down-regulated in pPROM with ACA than other groups. Nodal and cripto were not expressed by fetal membranes.

Conclusion: The study shows the involvement of activin A pathway in pPROM with ACA. Further studies will focus on its role in placental immune functions.

MeSH terms

  • Activins / genetics
  • Activins / metabolism*
  • Chorioamnionitis / metabolism*
  • Down-Regulation
  • Extraembryonic Membranes / metabolism*
  • Female
  • Fetal Membranes, Premature Rupture / metabolism*
  • Follistatin / genetics
  • Follistatin / metabolism
  • GPI-Linked Proteins / genetics
  • GPI-Linked Proteins / metabolism
  • Gene Expression Regulation*
  • Humans
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism
  • Nodal Protein / genetics
  • Nodal Protein / metabolism
  • Placenta / metabolism*
  • Pregnancy
  • Pregnancy Complications / metabolism
  • Premature Birth
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Up-Regulation

Substances

  • Follistatin
  • GPI-Linked Proteins
  • Intercellular Signaling Peptides and Proteins
  • NODAL protein, human
  • Neoplasm Proteins
  • Nodal Protein
  • RNA, Messenger
  • TDGF1 protein, human
  • activin A
  • Activins

Supplementary concepts

  • Preterm Premature Rupture of the Membranes