Understanding heart metabolism during preservation is crucial to develop new effective cardioplegic solutions. We aim to investigate metabolic alterations during heart preservation in the clinically used Celsior (Cs) and histidine buffer solution (HBS). We also focused in gender-specific metabolic adaptations during ischemia. We followed energy metabolism in hearts from males and females preserved during 6 hours in Cs and HBS. Hearts were subjected to cold ischemia (4°C) in Cs or HBS, and aliquots of the cardioplegic solution were collected throughout preservation for nuclear magnetic resonance analysis. HBS-preserved hearts from males consumed glucose mostly between 240 and 360 minutes, whereas HBS-preserved hearts from females consumed glucose throughout the 6 hours of ischemia. Lactate production rates followed approximately the glucose consumption rates in HBS-preserved hearts. The lactate to alanine ratio, an indicator of the redox state, was increased in HBS-preserved hearts when compared with Cs-preserved hearts. Hearts from males presented a higher redox state than those from females preserved in Cs after 300 minutes. Both Cs and HBS were capable of preventing acidification in hearts from females but not in hearts from males, which decreased the extracellular pH. HBS-preserved hearts from males and females produced 0.1 ± 0.01 and 0.15 ± 0.03 μmol·min·gdw of lactate, respectively. Those rates were significantly higher than in Cs-preserved hearts. Thus, Cs was more effective in preventing lactate production. We conclude that glycolysis and lactate production are stimulated in HBS-preserved hearts. HBS shows better overall results particularly in hearts from females, which presented less extracellular acidification and were more effective in recycling the metabolic subproducts.