Bivalirudin utilization in rats undergoing cardiopulmonary bypass: preventing the increase of antiheparin/platelet factor 4 antibody in perioperative period

Clin Appl Thromb Hemost. 2014 Mar;20(2):196-8. doi: 10.1177/1076029612456735. Epub 2012 Aug 21.

Abstract

Objective: To investigate the impact of bivalirudin alternative treatment on antiheparin/platelet factor 4 antibody levels perioperatively in rats undergoing cardiopulmonary bypass (CPB).

Methods: Two groups of rats received heparin pretreatment and two groups of rats not, and during CPB all the four groups received either bivalirudin or heparin. Pre-CPB and post-CPB day 7 venous blood samples were used to determine the levels of antiheparin/PF4 antibody immunoglobulin (Ig) G with enzyme-linked immunosorbent assay.

Results: Preoperative heparin pretreatment increased the antiheparin/PF4 antibody IgG levels (P < .01), while the administration of heparin further increased this levels (P < .01). The use of bivalirudin prevented further increase in antiheparin/PF4 antibody IgG levels.

Conclusion: Bivalirudin, as a direct thrombin inhibitor, could circumvent the increased antiheparin/PF4 antibody level in circulation due to the heparin administration.

Keywords: antiheparin/platelet factor 4 antibody; bivalirudin; cardiopulmonary bypass; unfractionated heparin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticoagulants / pharmacology*
  • Antithrombins / pharmacology*
  • Autoantibodies / immunology
  • Autoantibodies / metabolism*
  • Cardiopulmonary Bypass / methods*
  • Heparin / immunology
  • Heparin / pharmacology
  • Hirudins / pharmacology*
  • Immunoglobulin G / immunology
  • Immunoglobulin G / metabolism
  • Male
  • Peptide Fragments / pharmacology*
  • Platelet Factor 4 / immunology*
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Proteins / pharmacology

Substances

  • Anticoagulants
  • Antithrombins
  • Autoantibodies
  • Hirudins
  • Immunoglobulin G
  • Peptide Fragments
  • Recombinant Proteins
  • Platelet Factor 4
  • Heparin
  • bivalirudin