Effects of rs7903146 variation in the Tcf7l2 gene in the lipid metabolism of three different populations

PLoS One. 2012;7(8):e43390. doi: 10.1371/journal.pone.0043390. Epub 2012 Aug 20.

Abstract

Background: TCF7L2 rs7903146 is an important genetic factor predicting type 2 diabetes (T2DM) which has also been linked to higher cardiovascular risk. To date, there is little information about the additional impact of this single nucleotide polymorphism (SNP) beyond glucose metabolism.

Methodology/principal findings: We studied whether rs7903146 influenced postprandial lipid metabolism in three different populations (healthy young men, metabolic syndrome (MetS) patients and elderly persons). Eighty-eight healthy males were submitted to a single saturated fatty acid-rich test meal. Additionally, 110 middle-aged MetS patients and 20 healthy elderly persons (≥ 65 years) were submitted to three different dietary models followed by test meals. Minor allele homozygotes for rs7903146 showed a worse postprandial lipemia profile in young males, as seen by a lower HDL-cholesterol and Apo A1 concentration during the postprandial lipemia and a trend towards higher triglycerides (TG), than the other genotypes. In healthy elderly persons, carriers of the minor allele showed higher total cholesterol, LDL-cholesterol, Apo B and TG in the fasting state, and a higher postprandial area under the curve for total cholesterol, Apo B, small-triglyceride rich lipoprotein (TRL) cholesterol and small-(TRL) triglycerides. These results were accompanied by differential changes in adipokines. We did not observe any influence of rs7903146 on the postprandium of MetS patients.

Conclusions/significance: Healthy young males and elderly persons who are carriers of the mutant allele for rs7903146 have an impaired postprandial lipid metabolism that may be mediated by an alteration in adipokine regulation, and may be related to the higher cardiovascular risk observed in these persons.

Trial registration: ClinicalTrials.gov NCT00429195.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Cholesterol / metabolism
  • Genetic Variation / genetics*
  • Humans
  • Lipid Metabolism / genetics*
  • Lipoproteins / metabolism
  • Male
  • Polymorphism, Single Nucleotide / genetics
  • Transcription Factor 7-Like 2 Protein / genetics*
  • Triglycerides / metabolism
  • Young Adult

Substances

  • Lipoproteins
  • TCF7L2 protein, human
  • Transcription Factor 7-Like 2 Protein
  • Triglycerides
  • Cholesterol

Associated data

  • ClinicalTrials.gov/NCT00429195

Grants and funding

Supported in part by public funds: research grants from the Spanish Ministry of Science and Innovation (AGL 2004-07907, AGL2006-01979, and AGL2009-12270 to JL-M, SAF07-62005 to FP-J and FIS PI10/01041 to PP-M, PI10/02412 to FP-J); Consejería de Economía, Innovación y Ciencia, Proyectos de Investigación de Excelencia, Junta de Andalucía (P06-CTS-01425 to JL-M, CTS5015 and AGR922 to FP-J); Consejería de Salud, Junta de Andalucía (06/128, 07/43, and PI0193/09 to JL-M, 06/129 to FP-J, 0118/08 to FF-J, PI-0252/09 to JD-L, and PI-0058/10 to PP-M); Fondo Europeo de Desarrollo Regional (FEDER). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.