Rapid detection of chimeric bcr/abl mRNAs in acute lymphoblastic and chronic myeloid leukemia by the polymerase chain reaction

Blut. 1990 Dec;61(6):350-3. doi: 10.1007/BF01738548.

Abstract

We have developed a rapid method for the detection of bcr/abl mRNAs, the products of the BCR/ABL fusion genes. The method is based on the polymerase-chain-reaction (PCR). Through the use of additional internal primers it is possible to detect directly a single Ph1-positive cell among 10(5) unaffected cells thus omitting time-consuming blotting procedures. The whole analytical procedure starting from RNA isolation to agarose gel electrophoresis including two rounds of PCR can be performed in less than six hours.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Fusion Proteins, bcr-abl / genetics*
  • Humans
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Molecular Sequence Data
  • Philadelphia Chromosome
  • Polymerase Chain Reaction*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • RNA, Messenger / analysis*

Substances

  • RNA, Messenger
  • Fusion Proteins, bcr-abl