Fetal growth restriction in hypothyroidism is associated with changes in proliferative activity, apoptosis and vascularisation of the placenta

Reprod Fertil Dev. 2012;24(7):923-31. doi: 10.1071/RD11219.

Abstract

The objective of this study was to evaluate fetal weight, histomorphometric changes and proliferative activity, apoptosis and angiogenesis of the placenta in rats with hypothyroidism. Thirty-six adult female rats were divided into two groups with 18 animals each: control and hypothyroidism. Hypothyroidism was induced by daily administration of propylthiouracil (1 mg/animal). The administration began five days before becoming pregnant and the animals were sacrificed at 14 or 19 days of gestation. The control group received a placebo. The number and weight of fetuses and the rate of fetal death was determined, as well as the morphometric characteristics, the immunohistochemical expression of cell division control protein 47 (CDC)-47 and vascular endothelial growth factor (VEGF) and the number of apoptotic cells in the placental disk. The data were analysed by Mann-Whitney U test. Hypothyroidism reduced the weight of fetuses and of the uterus and placenta (P<0.05), altered the thickness of the placental labyrinth and spongiotrophoblast (P<0.05), increased the population of glycogen cells in the spongiotrophoblast (P<0.05), interfered with the vascular development of the placental labyrinth and decreased VEGF expression (P<0.05), reduced the expression of CDC-47 and cellularity and increased the apoptotic rate in the placental disk (P<0.05). We conclude that hypothyroidism affects fetal weight by altering the proliferative activity, apoptosis and vascularisation of the placenta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Animals
  • Apoptosis*
  • Biomarkers / metabolism
  • Cell Proliferation*
  • DNA-Binding Proteins / metabolism
  • Disease Models, Animal
  • Down-Regulation
  • Female
  • Fetal Death
  • Fetal Growth Retardation / etiology*
  • Fetal Growth Retardation / metabolism
  • Fetal Growth Retardation / pathology
  • Fetal Growth Retardation / physiopathology
  • Fetal Weight
  • Gestational Age
  • Glycogen / metabolism
  • Hypothyroidism / chemically induced
  • Hypothyroidism / complications*
  • Immunohistochemistry
  • Minichromosome Maintenance Complex Component 7
  • Neovascularization, Physiologic*
  • Placenta / blood supply*
  • Placenta / metabolism
  • Placenta / pathology*
  • Pregnancy
  • Propylthiouracil
  • Rats
  • Trophoblasts / metabolism
  • Trophoblasts / pathology
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Biomarkers
  • DNA-Binding Proteins
  • Vascular Endothelial Growth Factor A
  • vascular endothelial growth factor A, rat
  • Propylthiouracil
  • Glycogen
  • Adenosine Triphosphatases
  • MCM7 protein, rat
  • Minichromosome Maintenance Complex Component 7