How to investigate interactions between membrane proteins and ligands by solid-state NMR

Methods Mol Biol. 2012:914:65-86. doi: 10.1007/978-1-62703-023-6_5.

Abstract

Solid-state NMR is an established method for biophysical studies of membrane proteins within the lipid bilayers and an emerging technique for structural biology in general. In particular magic angle sample spinning has been found to be very useful for the investigation of large membrane proteins and their interaction with small molecules within the lipid bilayer. Using a number of examples, we illustrate and discuss in this chapter, which information can be gained and which experimental parameters need to be considered when planning such experiments. We focus especially on the interaction of diffusive ligands with membrane proteins.

Publication types

  • Review

MeSH terms

  • Humans
  • Ligands
  • Magnetic Resonance Spectroscopy / methods*
  • Membrane Proteins / metabolism*
  • Models, Molecular
  • Protein Binding
  • Receptor, Bradykinin B2 / chemistry
  • Receptor, Bradykinin B2 / metabolism

Substances

  • Ligands
  • Membrane Proteins
  • Receptor, Bradykinin B2