Curcuma longa extract exerts a myorelaxant effect on the ileum and colon in a mouse experimental colitis model, independent of the anti-inflammatory effect

PLoS One. 2012;7(9):e44650. doi: 10.1371/journal.pone.0044650. Epub 2012 Sep 12.

Abstract

Background: Curcuma has long been used as an anti-inflammatory agent in inflammatory bowel disease. Since gastrointestinal motility is impaired in inflammatory states, the aim of this work was to evaluate if Curcuma Longa had any effect on intestinal motility.

Methods: The biological activity of Curcuma extract was evaluated against Carbachol induced contraction in isolated mice intestine. Acute and chronic colitis were induced in Balb/c mice by Dextran Sulphate Sodium administration (5% and 2.5% respectively) and either Curcuma extract (200 mg/kg/day) or placebo was thereafter administered for 7 and 21 days respectively. Spontaneous contractions and the response to Carbachol and Atropine of ileum and colon were studied after colitis induction and Curcuma administration.

Results: Curcuma extract reduced the spontaneous contractions in the ileum and colon; the maximal response to Carbachol was inhibited in a non-competitive and reversible manner. Similar results were obtained in ileum and colon from Curcuma fed mice. DSS administration decreased the motility, mainly in the colon and Curcuma almost restored both the spontaneous contractions and the response to Carbachol after 14 days assumption, compared to standard diet, but a prolonged assumption of Curcuma decreased the spontaneous and Carbachol-induced contractions.

Conclusions: Curcuma extract has a direct and indirect myorelaxant effect on mouse ileum and colon, independent of the anti-inflammatory effect. The indirect effect is reversible and non-competitive with the cholinergic agent. These results suggest the use of curcuma extract as a spasmolytic agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Arrhythmia Agents / pharmacology
  • Anti-Inflammatory Agents / pharmacology*
  • Atropine / pharmacology
  • Carbachol / pharmacology
  • Cardiotonic Agents / pharmacology
  • Colitis / drug therapy*
  • Colon / drug effects*
  • Curcuma / metabolism*
  • Ileum / drug effects*
  • Inflammation / pathology
  • Intestines / drug effects
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Muscle Relaxants, Central / pharmacology
  • Plant Extracts / pharmacology*

Substances

  • Anti-Arrhythmia Agents
  • Anti-Inflammatory Agents
  • Cardiotonic Agents
  • Muscle Relaxants, Central
  • Plant Extracts
  • Atropine
  • Carbachol

Grants and funding

This work was supported by grants from Fondazione del Monte di Bologna e Ravenna and from Alisal (Mazz100136- 2010). ALISAL is a project supported by the MINISTERO DELLE POLITICHE AGRICOLE, ALIMENTARI E FORESTALI, with D.M. 11008/7303/10, dated 17/05/2010. The title of the project is: Miglioramento delle proprietà igienico-sanitarie, salutistiche e funzionali di commodity per l’alimentazione dell’uomo e/o degli animali (Improvement of sanitary properties, healthy and functional food commodities for human and/or animals). Prof. Mazzella was supported with materials, reagents, animals. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.