Human myxovirus resistance protein 1 (MxA) as a useful marker in the differential diagnosis of subcutaneous lymphoma vs. lupus erythematosus profundus

Eur J Dermatol. 2012 Sep-Oct;22(5):629-33. doi: 10.1684/ejd.2012.1790.

Abstract

Background: Substantial clinicohistologic overlap exists between lupus erythematosus profundus (LEP) and lymphomas involving the subcutis, including subcutaneous panniculitis-like T-cell lymphoma (SPTCL) and primary cutaneous gamma/delta T-cell lymphoma (GDTCL). Unequivocal markers separating the entities are not established.

Objectives: To explore the usefulness of interferon alpha (INF-α)-induced protein, myxovirus resistance protein 1 (MxA), in the differential diagnosis of these entities, as studies show that the expression pattern of MxA follows the distribution of the inflammatory infiltrate in cutaneous lupus, while INF- α is not known to operate in lymphoma.

Materials and methods: MxA immunohistochemistry was performed on skin biopsies from 5 patients with a clinical and histological diagnosis of SPTCL, 9 patients with GDTCL and 9 patients with LEP.

Results: In SPTCL and GDTCL, MxA was primarily seen in macrophages and generally did not exceed 20% of the infiltrate. In contrast, a significant portion of the subcutaneous infiltrate was positive for MxA in LEP, with 50% of the infiltrate staining on average. A greater number of macrophages and lymphocytes stained with a greater intensity as well (P<0.001). Moreover, endothelial cell staining was uniquely identified in LEP but not in lymphoma.

Conclusion: Although specificity is not 100%, minimal staining of MxA is a predictor for SPTCL or GDTCL. Conversely, extensive staining for MxA both qualitatively and quantitatively is a feature of LEP. Endothelial staining also appears to be specific for LEP.

MeSH terms

  • Biomarkers / metabolism
  • Diagnosis, Differential
  • GTP-Binding Proteins / metabolism*
  • Humans
  • Immunohistochemistry
  • Lymphocytes / metabolism
  • Lymphoma, T-Cell / diagnosis
  • Lymphoma, T-Cell / metabolism*
  • Lymphoma, T-Cell, Cutaneous / diagnosis
  • Lymphoma, T-Cell, Cutaneous / metabolism*
  • Macrophages / metabolism
  • Myxovirus Resistance Proteins
  • Panniculitis / diagnosis
  • Panniculitis / metabolism*
  • Panniculitis, Lupus Erythematosus / diagnosis
  • Panniculitis, Lupus Erythematosus / metabolism*
  • Skin Neoplasms / diagnosis
  • Skin Neoplasms / metabolism*

Substances

  • Biomarkers
  • MX1 protein, human
  • Myxovirus Resistance Proteins
  • GTP-Binding Proteins

Supplementary concepts

  • Subcutaneous panniculitis-like T-cell lymphoma