Click and pick: identification of sialoside analogues for siglec-based cell targeting

Angew Chem Int Ed Engl. 2012 Oct 29;51(44):11014-8. doi: 10.1002/anie.201205831. Epub 2012 Oct 4.

Abstract

Click 'n' chips: Azide and alkyne-bearing sialic acids (purple diamond; see picture) were subjected to high-throughput click chemistry to generate a library of sialic acid analogues. Microarray printing of the library and screening with the siglec family of sialic-acid-binding proteins, led to the identification of high-affinity ligands for siglec-9 and siglec-10.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Carbohydrate Conformation
  • Cell Line
  • Click Chemistry
  • Cricetinae
  • Humans
  • Leukocytes, Mononuclear / cytology*
  • Mice
  • N-Acetylneuraminic Acid / analogs & derivatives
  • N-Acetylneuraminic Acid / chemical synthesis
  • N-Acetylneuraminic Acid / chemistry*
  • Protein Array Analysis
  • Sialic Acid Binding Immunoglobulin-like Lectins / chemistry*

Substances

  • Sialic Acid Binding Immunoglobulin-like Lectins
  • N-Acetylneuraminic Acid