The placental growth factor as a target against hepatocellular carcinoma in a diethylnitrosamine-induced mouse model

J Hepatol. 2013 Feb;58(2):319-28. doi: 10.1016/j.jhep.2012.09.032. Epub 2012 Oct 6.

Abstract

Background & aims: The placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family known to stimulate endothelial cell growth, migration and survival, attract angiocompetent macrophages, and determine the metastatic niche. Unlike VEGF, genetic studies have shown that PlGF is specifically involved in pathologic angiogenesis, thus its inhibition would not affect healthy blood vessels, providing an attractive drug candidate with a good safety profile.

Methods: We assess whether inhibition of PlGF could be used as a potential therapy against hepatocellular carcinoma (HCC), by using PlGF knockout mice and monoclonal anti-PlGF antibodies in a mouse model for HCC. In addition, the effect of PlGF antibodies is compared to that of sorafenib, as well as the combination of both therapies.

Results: We have found that both in a transgenic knockout model and in a treatment model, targeting PlGF significantly decreases tumor burden. This was achieved not only by inhibiting neovascularisation, but also by decreasing hepatic macrophage recruitment and by normalising the remaining blood vessels, thereby decreasing hypoxia and reducing the prometastatic potential of HCC.

Conclusions: Considering the favourable safety profile and its pleiotropic effect on vascularisation, metastasis and inflammation, PlGF inhibition could become a valuable therapeutic strategy against HCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology
  • Antibodies, Monoclonal / therapeutic use
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents / therapeutic use
  • Carcinoma, Hepatocellular / chemically induced*
  • Carcinoma, Hepatocellular / drug therapy
  • Carcinoma, Hepatocellular / physiopathology*
  • Diethylnitrosamine / adverse effects*
  • Disease Models, Animal
  • Drug Therapy, Combination
  • Liver Neoplasms / chemically induced*
  • Liver Neoplasms / drug therapy
  • Liver Neoplasms / physiopathology*
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Neoplasm Metastasis / drug therapy
  • Neoplasm Metastasis / physiopathology
  • Neovascularization, Pathologic / drug therapy
  • Neovascularization, Pathologic / physiopathology
  • Niacinamide / analogs & derivatives
  • Niacinamide / pharmacology
  • Niacinamide / therapeutic use
  • Phenylurea Compounds / pharmacology
  • Phenylurea Compounds / therapeutic use
  • Placenta Growth Factor
  • Pregnancy Proteins / deficiency
  • Pregnancy Proteins / immunology
  • Pregnancy Proteins / physiology*
  • Sorafenib
  • Treatment Outcome

Substances

  • Antibodies, Monoclonal
  • Antineoplastic Agents
  • Pgf protein, mouse
  • Phenylurea Compounds
  • Pregnancy Proteins
  • Placenta Growth Factor
  • Niacinamide
  • Diethylnitrosamine
  • Sorafenib