Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)

J Med Primatol. 2012 Dec;41(6):407-11. doi: 10.1111/jmp.12024. Epub 2012 Oct 18.

Abstract

Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior.

Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data.

Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective.

Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Behavior, Animal*
  • DNA / analysis
  • Electrophoresis, Capillary / veterinary
  • Female
  • Genotyping Techniques / methods
  • Genotyping Techniques / veterinary*
  • Macaca mulatta / genetics*
  • Male
  • Monoamine Oxidase / genetics*
  • Polymerase Chain Reaction / veterinary
  • Polymorphism, Genetic / genetics*
  • Serotonin Plasma Membrane Transport Proteins / genetics*

Substances

  • Serotonin Plasma Membrane Transport Proteins
  • DNA
  • Monoamine Oxidase